What is AOD-9604?
AOD-9604 (CAS 221231-10-3) is a synthetic peptide belonging to the class of growth-hormone-derived fragment analogs. It corresponds to the C-terminal region of the human growth hormone molecule and carries the sequence Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe, comprising 16 amino acid residues. An N-terminal tyrosine is appended to the native fragment, a structural feature that distinguishes the analog from the unmodified parent sequence.
Structurally, the molecule is defined by the molecular formula C78H123N23O23S2 with an average molecular weight of 1815.08 g/mol. Its two cysteine residues at positions 7 and 15 form an intramolecular disulfide bridge (Cys7-Cys15), producing a constrained cyclic loop that is central to the peptide's conformation and to its analytical identity. The presence of arginine and glutamate residues contributes to its charge distribution and aqueous behavior in reconstituted form.
Within scientific literature, AOD-9604 is a compound studied in metabolic and lipid-metabolism research models as a fragment analog of the somatotropic hormone family. It is offered here strictly as a reference material for in-vitro and laboratory investigation. This listing characterizes the molecule itself — its classification, sequence, and physicochemical profile — and makes no representation regarding physiological activity or outcomes in humans or animals. The material is for research use only.
Reconstitution & handling
AOD-9604 is supplied as a lyophilized powder and is typically reconstituted with bacteriostatic or sterile water for laboratory handling; the disulfide-constrained peptide is generally soluble in aqueous buffers at neutral to slightly acidic pH. To preserve structural integrity, solvent should be introduced slowly against the wall of the vial and allowed to dissolve by gentle swirling rather than vigorous agitation or vortexing, which can introduce shear stress and foaming at the air-liquid interface. The Cys7-Cys15 disulfide bond makes the molecule sensitive to strongly reducing conditions, so reducing agents should be avoided unless a specific experimental protocol requires them.
For sparingly soluble handling scenarios, a minimal volume of dilute acetic acid can serve as an initial solubilizing vehicle before dilution into the working buffer, though a compatible aqueous diluent is sufficient for most preparations. Reconstituted solutions should be prepared in clean, low-binding labware, kept cold during manipulation, and used within the analytical workflow promptly to limit hydrolytic and oxidative changes to the peptide backbone and disulfide linkage.
Storage & stability
In its lyophilized state, AOD-9604 is stable for extended periods when stored sealed at -20 C, protected from light, moisture, and repeated temperature cycling; desiccated storage limits hydration-driven degradation of the powder. Once reconstituted, the peptide is less stable and should be held refrigerated at 2-8 C for short-term use or aliquoted and frozen at -20 C or below for longer intervals. Aliquoting prior to freezing is recommended to avoid repeated freeze-thaw cycles, which can promote aggregation, disulfide scrambling, and loss of chromatographic purity over time.
How it's tested
Identity and purity of AOD-9604 are established analytically prior to release. Reversed-phase high-performance liquid chromatography (HPLC) is used to quantify chromatographic purity, confirmed at ≥99% for this material, and to resolve related peptide impurities and truncation products. Mass spectrometry (MS) verifies molecular identity against the expected average mass of 1815.08 g/mol corresponding to the C78H123N23O23S2 composition, and confirms formation of the intramolecular Cys7-Cys15 disulfide. Each lot is accompanied by a Certificate of Analysis (COA) documenting the sequence, measured purity, and mass-confirmation data so researchers can verify material integrity before use.