What is PINEALON?
Pinealon is a synthetic tripeptide classified within the family of short peptide bioregulators. It is defined by the amino acid sequence Glu-Asp-Arg, commonly abbreviated EDR, comprising three residues linked by standard peptide bonds. The molecule carries the CAS registry number 175175-23-2, the molecular formula C15H26N6O8, and a molecular weight of 418.41 g/mol.
Structurally, the peptide pairs two acidic residues, glutamic acid (Glu) and aspartic acid (Asp), with the basic, guanidinium-bearing residue arginine (Arg). This combination of carboxylate side chains and a strongly basic terminal residue gives the molecule a zwitterionic, highly polar character at neutral pH, which informs its aqueous solubility and handling behavior in the laboratory. As a low-molecular-weight tripeptide, it lacks the higher-order folding of larger polypeptides and is handled as a defined, single-sequence chemical entity.
Pinealon belongs to the broader class of short peptides that are studied in vitro and in preclinical laboratory models within peptide-bioregulator research. It is used strictly as a reference material and experimental input for characterization, analytical method development, and related non-clinical investigation. This material is offered for laboratory research use only and is not a drug, supplement, or article intended for diagnostic or therapeutic use.
Reconstitution & handling
Pinealon is supplied as a lyophilized powder and is typically reconstituted for laboratory work using bacteriostatic or sterile water, which serves as the primary solvent for this polar tripeptide. Because the sequence contains two acidic residues and one basic residue, the peptide is generally freely soluble in aqueous media; the diluent should be added slowly down the side of the vial and allowed to dissolve without vigorous agitation. Gentle swirling rather than shaking helps preserve peptide integrity and avoids foaming or shear stress on the material.
If dissolution is incomplete, the vial can be left to stand at a low temperature to allow the solid to fully solvate, and mild warming to room temperature may assist. Reconstituted solutions should be prepared with analytical-grade water and buffers, kept at a defined pH near neutral where the peptide is most stable, and used within labware compatible with aqueous peptide handling. No dosing or administration information is provided; concentrations and volumes are determined solely by the researcher's in-vitro experimental design.
Storage & stability
In its lyophilized form, Pinealon is best stored sealed and protected from moisture and light at freezer temperatures (commonly -20 C or colder) to maximize long-term stability, and it remains stable for short intervals at refrigerated or ambient conditions during handling. Once reconstituted, the peptide should be kept refrigerated and used within a short working window; for extended storage, aliquoting the solution and freezing the fractions minimizes degradation and avoids repeated freeze-thaw cycles, which can compromise peptide integrity. Labeling each aliquot with concentration and preparation date supports reproducible laboratory practice.
How it's tested
Identity and purity of Pinealon are established by orthogonal analytical methods. Reversed-phase high-performance liquid chromatography (HPLC) confirms chromatographic purity of ≥99%, resolving the target peptide from process-related impurities, while mass spectrometry (MS) verifies molecular identity against the expected molecular weight of 418.41 for the C15H26N6O8 tripeptide. Each lot is accompanied by a Certificate of Analysis (COA) documenting the observed purity, mass confirmation, and appearance, providing traceable analytical evidence of the material's specification for research documentation.